GnRH主动免疫对公猪体内粪臭素代谢的影响

doi:10.11843/j.issn.0366-6964.2016.06.008

Abstract

Abstract:

The objective of the present study was to investigate the effects of active immunization against GnRH on skatole metabolism in boars.Thirty-six boars at the age of 10 weeks were randomly allocated to 3 groups.Included intact males(not administrated)，Immuno-castrates(immunized against GnRH at 10 and 18 weeks of age)，and Surgical castrates (surgically castrated at the age of one week).The hormone levels of testosterone and estradiol in plasma were determined by ELISA，the androstenone levels in plasma and androstenone，skatole levels in fat were determined by HPLC.The mRNA expression of skatole-metabolism genes in the liver were analyzed by real-time fluorescence quantitative PCR technique，and the protein expression of them were determined by ELISA.Active immunization against GnRH in boars resulted in a atrophy of testes and reduction of plasma testosterone levels (P<0.05).Plasma testosterone，androstenone，estradiol levels and fat androstenone and skatole levels in immunized boars were similar to that of surgical castrated boars (P>0.05)，in which were significantly lower than that of intact controls (P<0.05).In the liver，CAR COUP-TF1，CYP2E1，CYB5A，CYP2C49，GSTO2 mRNA expression in immune-castrated boars were similar to that of surgical castrated boars (P>0.05)，which were significantly higher than that of intact controls (P<0.05)；the CYP2A19 mRNA expression in surgical castrated pigs was higher than in intact controls (P<0.05)，while CYP2A19 mRNA expression in immunized boars was in between，which was significantly different from either surgical castrates or intact controls (P<0.05)，PXR and SULT1A1 mRNA expression were not significantly different among 3 groups (P>0.05).In the liver，the CYP2E1，CYB5A protein expression were highest for surgical castrated pigs and lowest for intact controls，with vaccinated pigs at an intermediate level；CYP2A19，CYP2C49，GSTO2 protein expression in surgical castrated boars were similar to that of surgical castrated boars (P>0.05)，in which both were significantly higher than intact controls(P<0.05)；but SULT1A1 protein expression had no significant difference among 3 groups (P>0.05).Active immunization against GnRH in boars decreased plasma testosterone，estradiol and androstenone levels，increased CYP450s and GSTO2 mRNA and protein expression and to reduce boar-taint by accelerating skatole metabolism in the liver.

CLC Number:

S828.3

SUI Fen-fen，HAN Xing-fa，CAO Xiao-han，DU Xiao-gang，MENG Feng-yan，CHU Ming-xing，ZENG Xian-yin. Effects of Active Immunization against GnRH on Skatole Metabolism in Male Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1140-1146.

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Effects of Active Immunization against GnRH on Skatole Metabolism in Male Pigs

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